Sadia Bawany - Senior Microbiologist
Isolation Of Naegleria Fowleri
Dr. Essa Laboratory and Diagnostic Centre
Naegleria fowleri a brain eating amoeba, climate sensitive or thermophilic amoeba found in environment including hot springs and warm surface water, lakes and river can cause Primary Amoebic Meningoencephalitis in humans. Naegleria fowlwri usually enters the brain and attacks the nervous system when infected water is ingested through the nasal cavity while bathing, swimming or making ablution.
The organism goes through three stages in its lifecycle: Cysts, Flagellates, and Trophozoites. 440 cases of Primary Amoebic Meningoencephalitis due to Naegleria fowleri were reported worldwide; only 8 survivors have been reported worldwide until 2005. 13 cases on N. fowleri Primary Amoebic Meningoencephalitis reported in Aga Khan University Hospital in 2011 in Karachi, Pakistan. Increased in PAM is due to high temperature, less level of chlorine in water. A survey of different Pools in Karachi determined that Pools can be colonized and may be an unrecognized source of this organism that could present a human health risk. 30 samples were taken from different Pools of Karachi. Naegleria fowleri was microscopically observed then cultured on Page’s Amoeba Saline (Non-Nutrient Agar) seeded with E.coli. 18 samples showed the presence of protozoa by Direct Microscopy.
Sadia Bawany, Msc in Microbiology , working in Dr Essa laboratory and diagnostic centre as a Microbiologist , performing all type of lab techniques such as sample inoculation, Microscopy, Staining, Watching AFB slides, Lawning, Drugs Sensing, Streaking, Blood Culturing on BACTEC 9240, Organism Identification, Media Preparation, Making Perfect Slides, Reporting etc.
Ms Naheed Afshan, M.Phil in Microbiology, chairperson of Microbiology department, Jinnah University for Women, Karachi, Pakistan.